Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA

JYH Lee; N Best; J McAuley; JL Porter; T Seemann; MB Schultz; M Sait; N Orlando; K Mercoulia; SA Ballard; J Druce; T Tran; MG Catton; MJ Pryor; HL Cui; A Luttick; S McDonald; A Greenhalgh; JC Kwong; NL Sherry; M Graham; T Hoang; M Herisse; SJ Pidot; DA Williamson; BP Howden; IR Monk; TP Stinear

Journal article

Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA

JYH Lee, N Best, J McAuley, JL Porter, T Seemann, MB Schultz, M Sait, N Orlando, K Mercoulia, SA Ballard, J Druce, T Tran, MG Catton, MJ Pryor, HL Cui, A Luttick, S McDonald, A Greenhalgh, JC Kwong, NL Sherry Show all

Journal of Medical Microbiology | MICROBIOLOGY SOC | Published : 2020

DOI: 10.1099/jmm.0.001238

Download PDF

Abstract

Introduction. The SARS-CoV-2 pandemic of 2020 has resulted in unparalleled requirements for RNA extraction kits and enzymes required for virus detection, leading to global shortages. This has necessitated the exploration of alternative diagnostic options to alleviate supply chain issues. Aim. To establish and validate a reverse transcription loop-mediated isothermal amplification (RT- LAMP) assay for the detection of SARS-CoV-2 from nasopharyngeal swabs. Methodology. We used a commercial RT-LAMP mastermix from OptiGene in combination with a primer set designed to detect the CDC N1 region of the SARS-CoV-2 nucleocapsid (N) gene. A single-tube, single-step fluorescence assay was implemented wh..

View full abstract

University of Melbourne Researchers

Jean Lee Author

Julie McAuley Author

Jessica Porter Author

Torsten Seemann Author

Related Projects (3)

GENOMIC APPROACHES IN HUMAN HEALTH MICROBIOLOGY

This project uses latest genomic technologies to advance our understanding of how bacteria cause disease and finding new antibiotics/approac..

IMPROVING PREVENTION, TRACKING AND TREATMENT OF MAJOR HUMAN BACTERIAL PATHOGENS

This research fellowship will lead to a detailed understanding of the mechanisms by which bacteria become resistant to antibiotics and cause..

APPLYING PATHOGEN GENOMICS TO THE PREVENTION AND CONTROL OF ANTIMICROBIAL RESISTANCE

Antimicrobial resistance (AMR) is a huge threat to public health, both in Australia and globally. In some instances, having an AMR infection..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

D.A.W. is supported by an Emerging Leadership Investigator Grant from the National Health and Medical Research Council (NHMRC) of Australia (APP1174555). T.P.S. is supported by NHMRC Research Fellowship (APP1105525). B.P.H. is supported by NHMRC Practitioner Fellowship (APP1105905).